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Fish scale derived hydroxyapatite scaffold for bone tissue engineering
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DOI：10.1016/j.matchar.2016.09.034.png)
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摘要
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The tailored mechanical characteristics of bioceramic scaffold are very important for the bone tissue engineering. Functional hydroxyapatite (HAp) synthesized from fish scale (Labio rohita & Cotta catla) is explored as scaffold materials. HAp scaffold exhibits better mechanical properties such as hardness (similar to 1.08 GPa), compressive strength (similar to 0.8 GPa) and tensile strength (similar to 187 MPa) with adequate porosity (similar to 35%) compared to commercial and synthetic body fluid derived HAp. The in vitro assays (MTT, LDH and Trypan blue) with MG63 osteoblast cell lines reveal that fish scale derived HAp materials is non-toxic and bioactive. The in vivo histological analysis of implanted HAp scaffold shows the osteo-conductive characteristics. The findings are significant with respect to cytotoxicity, osteo-conductivity and mechanical stability during bio-mineralization of traumatized bone tissues. (C) 2016 Elsevier Inc. All rights reserved.
Keyword:
Hydroxyapatite
Scaffold
Cytotoxicity
Cell-attachment
Sintering
Tissue-engineering
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1.1W
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3.4W
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