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Five novel Haloarchaeobius species from coastal tidal flats and saline-alkali soils in China using integrated culture-dependent and culture-independent approaches
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DOI：10.1016/j.syapm.2026.126718.png)
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Abstract
En 中文
Six novel halophilic archaeal strains, DFWS5T, DT45T, DYHT-AS-18T, HRN-SO-5T, TZWSO28, and TZWWS8T were isolated from tidal flats and saline-alkali soil collected from the eastern coastal region of China. Amplicon sequencing and metagenomic analyses indicated that these strains were present at low abundance in their original habitats, with only three strains detected by culture-independent approaches. These six strains constituted an independent clade alongside members of the genus Haloarchaeobius based on the 16S rRNA gene phylogeny. Except for the comparison between strains DYHT-AS-18T and TZWSO28, the average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values among these strains and existing members of the genus Haloarchaeobius were 76.60–89.50%, 21.00–38.50%, and 67.99–88.76%, respectively, below the proposed thresholds for species delineation. In contrast, these three values between strains DYHT-AS-18T and TZWSO28 were 97.36%, 76.30%, and 97.25%, respectively, exceeding the proposed thresholds. Phylogenomic analysis revealed that the six strains clustered with members of the genus Haloarchaeobius, but formed distinct branches separate from the current species. The optimal growth conditions for these six strains in terms of NaCl, MgCl2, temperature, and pH were 0.9–4.8 M, 0–1 M, 20–50 °C, and 5.0–9.5, respectively. According to phenotypic differences in nutrition and biochemical activity, these six strains can be distinguished from their related species. On the basis of polyphasic taxonomic evidence, five novel species within the genus Haloarchaeobius are proposed to accommodate strains DFWS5T, DT45T, DYHT-AS-18T, HRN-SO-5T, TZWSO28, and TZWWS8T, respectively.
Keywords:
Haloarchaeobius
novel species
halophilic archaea
16S rRNA gene phylogeny
polyphasic taxonomy
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