.png){kind=icon}
返回
Flap Endonuclease 1
.png){kind=icon}
OA
AI
DOI：10.1146/annurev-biochem-072511-122603.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
First discovered as a structure-specific endonuclease that evolved to cut at the base of single-stranded flaps, flap endonuclease (FEN1) is now recognized as a central component of cellular DNA metabolism. Substrate specificity allows FEN1 to process intermediates of Okazaki fragment maturation, long-patch base excision repair, telomere maintenance, and stalled replication fork rescue. For Okazaki fragments, the RNA primer is displaced into a 5' flap and then cleaved off. FEN1 binds to the flap base and then threads the 5' end of the flap through its helical arch and active site to create a configuration for cleavage. The threading requirement prevents this active nuclease from cutting the single-stranded template between Okazaki fragments. FEN1 efficiency and specificity are critical to the maintenance of genome fidelity. Overall, recent advances in our knowledge of FEN1 suggest that it was an ancient protein that has been fine-tuned over eons to coordinate many essential DNA transactions.
Keyword:
endonucleases
genome fidelity
genome stability
long-patch base excision repair
Okazaki fragment maturation
期刊
IF:
20.5
论文数:
2.2K
被引数:
2.1W
机构
引用论文
Protective Effect of the HLA-DRB1*13:02 Allele in Japanese Rheumatoid Arthritis Patients.png)
PLoS ONE
OAAI
PLoS ONEIF0
OAAI分享收藏Sequential Posttranslational Modifications Program FEN1 Degradation during Cell-Cycle ProgressionMOLECULAR CELLOAAI
MOLECULAR CELLIF16.6
OAAI分享收藏分享收藏
查看更多内容