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Graded levels of wheat oligopeptides relieve feed change-induced intestinal damage via modulation of the Wnt/β-catenin signaling pathway in broilers
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DOI：10.1016/j.aninu.2026.03.004.png)
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Abstract
En 中文
The abrupt transition in feed composition and form during the starter to grower phase can damage intestinal barriers and affect broiler performance, triggering the feed change stress. This study aimed to elucidate the impact of feed change stress and investigate the effect and mechanism of wheat oligopeptides (WOP) on the intestinal damage induced by feed change stress in broilers. A total of 120 one-d-old male Arbor Acre broilers (initial body weight [BW] 45.38 ± 0.64 g) were randomly allocated into two treatments (feed change or not) with 6 replicates of 10 birds, with monitoring for 60 h after changing feeds on d 11. A total of 240 one-d-old male Arbor Acre broilers (initial BW 45.33 ± 0.55 g) were randomly assigned to four treatments supplemented with graded levels of WOP (0, 0.1%, 0.2%, and 0.3%) with 6 replicates of 10 birds. The experimental period was 42 d. Compared with the no feed change (FC) group, broilers exhibited decreased feed intake after 4 h of changing feeds (P = 0.006), increased jejunal permeability with lower claudin-2 expression, elevated IL-8, and TNF-α expression (P < 0.001), suppressed intestinal stem cell (ISC) differentiation evidenced by reduced Bmi1, Lgr5, Chga, SI, and Muc2 expression (P < 0.05). The stress induced by changing feeds in broilers may gradually diminish over time. The stress induced by changing feeds in broilers may gradually diminish over time. Dietary WOP supplementation significantly improved growth performance of broilers. Compared with the control group, broilers fed diets supplemented with WOP showed higher BW at d 42 (P = 0.010), greater average daily gain (ADG) during d 11–42 (P = 0.010), and lower feed/gain ratio (F/G) during d 11–42 (P = 0.002), with 0.2% WOP being the optimal inclusion level. Mechanistically, WOP promoted ISC differentiation, partly by modulating the Wnt/β-catenin pathway, evidenced by upregulated Wnt, β-catenin, and c-Myc mRNA and protein levels (P < 0.05). Notably, WOP counteracted feed change-induced suppression of nutrient transporters and inflammatory responses. These findings indicate that changing feeds impairs intestinal health via ISC dysregulation, while WOP alleviates these effects by modulating ISC differentiation and the Wnt/β-catenin signaling pathway.
Keywords:
Broiler
Feed change stress
Wheat oligopeptide
Intestinal stem cell
Wnt/β-catenin signaling pathway
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