.png){kind=icon}
返回
Gradient elution techniques for capillary electrochromatography
.png){kind=icon}
PRE
AI
DOI：10.1016/S0021-9673(00)00534-3.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Capillary electrochromatography (CEC) is a rapidly maturing technique, but still in need of further instrumental development and in need of unique applications that are not possible by traditional pressure-driven LC. We review the development of gradient elution schemes for CEC, beginning with pH gradients initially developed for capillary electrophoresis. Step gradients are the most easily instrumentally implemented, but provide less flexibility in separation than continuous gradients. Pressure-assisted CEC is easily adapted to gradient elution schemes, but does not offer the advantages of very high column efficiency provided by totally electro-driven mobile phases. The development of flow-injection interfaces allows a true solvent gradient to be generated by Ir-LC pumps, with the mobile phase drawn into the separation capillary by pure electroosmotic flow. While requiring both a CEC instrument and a traditional pump or pumps capable of generating the gradient, this method offers advantages of greatly reduced column handling, prolonging column lifetimes, and allows simple autosampling. We also discuss voltage gradients, which provide a mobile phase velocity gradient. (C) 2000 Elsevier Science B.V. All rights reserved.
Keyword:
gradient elution
electrochromatography
reviews
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
4
论文数:
3.2W
被引数:
5.0W
机构
暂无机构信息
引用论文
POU-domain factor expression in the trigeminal ganglion and implications for herpes virus regulation.png)
NeuroReport
NeuroReportIF0
PREAI
分享收藏Integrated microchip device with electrokinetically controlled solvent mixing for isocratic and gradient elution in micellar electrokinetic chromatographyANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏Long-Term No-Tillage Direct Seeding Mode for Water-Saving and Drought-Resistance Rice Production in Rice-Rapeseed Rotation SystemRice Science
Rice ScienceIF0
PREAI
分享收藏
分享收藏分享收藏
查看更多内容