High-cell-density culture for recombinant xylanase production and its application in hydrolysis of mild alkaline pretreated rice straw for xylooligosaccharide production

Tunyaboon Laemthong

doi:10.1016/j.procbio.2026.05.015

High-cell-density culture for recombinant xylanase production and its application in hydrolysis of mild alkaline pretreated rice straw for xylooligosaccharide production

2026-05-27

0

PRE

AI

T

Tunyaboon Laemthong

W

Wasana Sukhumsirichart

S

Supenya Chittapun

C

Chatchol Kongsinkaew

A

Atikant Satipattarn

A

Arachaporn Thong-olran

S

Soisuda Pornpukdeewattana

T

Theppanya Charoenrat *

DOI：10.1016/j.procbio.2026.05.015

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Abstract

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• Recombinant xylanase production was integrated for rice straw XOS. • Fed-batch E. coli cultivation yielded xylanase activity of 207.95 U/mL. • Mild alkaline pretreatment was optimized using response surface methodology. • Hydrolysis produced 6.13 mg/mL XOS, with X2 and X3 as dominant fractions. • Linking enzyme supply with pretreatment improved process consistency.

Keywords:

xylanase

rice straw

xylooligosaccharide

fed-batch cultivation

mild alkaline pretreatment

High-cell-density culture for recombinant xylanase production and its application in hydrolysis of mild alkaline pretreated rice straw for xylooligosaccharide production

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