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High-throughput biomolecular interaction analysis probing by an array fluorescent biosensor platform
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DOI：10.1016/j.snb.2017.12.119.png)
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Interactions between biomolecules have been extensively investigated in versatile applications, such as biosensing, medical diagnosis and drug screening. In this study, an improved biomolecular interaction analysis probing with an integrated array fluorescent biosensor platform is proposed to overcome the limitations of surface plasmon resonance (SPR), such as a small number of reactions that can be simultaneously analysed and weak reusability of biospecific active surfaces. Reactions between biological molecules at twenty-four individual biosensing spots are monitored in real time by measuring the changes in fluorescence intensity excited by an evanescent wave field at a biospecific active surface. A sensor chip with the biospecific active surface is reusable with over 200 test cycles. The interaction between bisphenol A (BPA) and anti-BPA antibody is chosen as a paradigm to validate this assay. With a wide range of low to high concentrations of anti-BPA antibody, twenty-four response curves at the individual biosensing spots can be generated in real time and simultaneously provide further information about the interactions between antibody and surface-tethered antigen. This system is also characterised by its high-throughput cross-reactivity analysis between antigens and antibodies on the patterned waveguide with a shortened time-to-result. (C) 2017 Elsevier B.V. All rights reserved.
Keyword:
Biomolecular interaction analysis
Array biosensor
Evanescent wave
Fluorescence
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7.7
论文数:
3.4W
被引数:
12.6W
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