Highly turn-on small-molecule fluorescent probe based on novel xanthene-derived skeleton for cysteine sensing in living cells, plants, and foods

2026-08-11

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Hai Yan Yuan

Ziyi Xu *

罗红群 (Hong Qun Luo) *

李念兵 (Nian Bing Li) *

DOI：10.1016/j.snb.2026.140679

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• Ring-expanded α,β-unsaturated ketone is designed as selective Cys response site. • Py-xan exhibits superb fluorescence enhancement (> 300 folds) for Cys detection. • Broad linear range (0.5-250 μM) detection of Cys is achieved by the Py-xan. • This fluorescent probe is applied to Cys imaging in multiple living systems.

Highly turn-on small-molecule fluorescent probe based on novel xanthene-derived skeleton for cysteine sensing in living cells, plants, and foods

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