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Hydrodynamic optical alignment for microflow cytometry

delete2011-01-01
delete34
delete
OA
AI
M
Matthew J. Kennedy
S
Scott Stelick
L
Lavanya G. Sayam
A
Andrew Yen
D
David Erickson
C
Carl A. Batt *
DOI：10.1039/c0lc00500bdelete
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摘要

摘要

En 中文
A microfabricated flow cytometer has been developed that is capable of detecting nearly all of the microparticles in an aqueous suspension. Current design allows for integrated coupling between an optical fiber-based detection system and the particle stream via hydrodynamic focusing. By adjusting the relative flow-rates at the auxiliary inputs of the focusing manifold, the particle stream can be steered out-of-plane relative to the illuminating laser, and similarly the particle stream can be squeezed or expanded. The microfabricated device was constructed in polydimethylsiloxane with cross-sectional microfluidic dimensions of 125 mu m x 125 mu m. Using the present device and method, fluorescent microparticles in aqueous solution were counted at an absolute counting efficiency of 91 +/- 4%. The coefficient of variation of the fluorescence pulse-heights for far-red fluorescent microparticles was 15%. The device exhibited a linear response to fluorescence intensity calibration microparticles as shown by comparison with a commercial cytometer instrument.
Keyword:
FLOW-CYTOMETRY
HIGH-THROUGHPUT
MANIPULATION
DEVICES
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期刊

L
Lab on a Chip
IF:
5.4
论文数:
9.0K
被引数:
3.3W

机构

C
Cornell University
学者数:
6.3W
论文数: 5.4W
被引数: 10.9W
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