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In Vitro and Embryonic Evaluation of Recombinant Larus ridibundus IFN-α and Mx Proteins Against Newcastle Disease Virus
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DOI：10.3390/pathogens15080818.png)
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Abstract
En 中文
Newcastle disease virus (NDV) is an important avian pathogen that can circulate in migratory birds, including Larus ridibundus. This study examined the transcriptional responses and anti-NDV activities associated with recombinant IFN-α and Mx proteins from L. ridibundus. The IFN-α and Mx open reading frames were cloned (GenBank: OP263971 and OP263970), expressed using a pET32a(+)/E. coli system, and assessed using chicken embryos, DF-1 cells, and primary gull lymphocytes. qRT-PCR analysis showed that NDV infection increased IFN-α and Mx transcript abundance in gull lymphocytes, with peak levels at 48 h post-infection (p < 0.01). In chicken embryos, recombinant IFN-α was associated with higher embryo survival and lower NDV NP transcript abundance than recombinant Mx under the tested conditions. In DF-1 cells and primary gull lymphocytes, 0.25 mg/mL recombinant IFN-α was associated with reduced NDV NP transcript abundance, and morphological inspection suggested less severe cytopathic effects in DF-1 cells. IFN-α treatment was also accompanied by lower transcription of TLR7, MyD88, IRF7, and Mx compared with the NDV group at 48 h. These data support a role for gull IFN-α in limiting NDV-associated transcriptional responses in vitro, while further protein-level and infectious-virus assays are required to define the underlying mechanism.
Keywords:
<i>Larus ridibundus</i>
Newcastle disease virus
IFN-α
Mx
qRT-PCR
innate antiviral immunity
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