.png){kind=icon}
返回
Insulin Aggregation at a Dynamic Solid-Liquid-Air Triple Interface
.png){kind=icon}
PRE
AI
DOI：10.1021/acs.langmuir.6b03314.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Therapeutic proteins are privileged in drug development because of their exquisite specificity, which is due to their three-dimensional conformation in solution. During their manufacture, storage, and delivery, interactions with material surfaces and air interfaces are known to affect their stability. The growing use of automated devices for handling and injection of therapeutics increases their exposure to protocols involving intermittent wetting, during which the solid-liquid and liquid-air interfaces meet at a triple contact line, which is often dynamic. Using a microfluidic setup, we analyze the effect of a moving triple interface on insulin aggregation in real time over a hydrophobic surface. We combine thioflavin T fluorescence and reflection interference microscopy to concomitantly monitor insulin aggregation and the morphology of the liquid as it dewets the surface. We demonstrate that insulin aggregates in the region of a moving triple interface and not in regions submitted to hydrodynamic shear stress alone, induced by the moving liquid. During dewetting, liquid droplets form on the surface anchored by adsorbed proteins, and the accumulation of amyloid aggregates is observed exclusively as fluorescent rings growing eccentrically around these droplets. The fluorescent rings expand until the entire channel surface sweeped by the triple interface is covered by amyloid fibers. On the basis of our experimental results, we propose a model describing the growth mechanism of insulin amyloid fibers at a moving triple contact line, where proteins adsorbed at a hydrophobic surface are exposed to the liquid-air interface.
Keyword:
WATER-INTERFACE
HYDROPHOBIC SURFACES
PROTEIN ADSORPTION
MONOCLONAL-ANTIBODY
HIGH-SHEAR
STABILIZATION
FORMULATIONS
SPECTROSCOPY
MICROSCOPY
MONOLAYER
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.9
论文数:
5.4W
被引数:
10.6W
机构
引用论文
Stygofaunal community trends along varied rainfall conditions: Deciphering ecological niche dynamics of a shallow calcrete in Western Australia.png)
Ecohydrology
OAAI
EcohydrologyIF0
OAAI分享收藏The Regulation of Pharmacists in Belgium and the Netherlands: In the Public or Private Interest?比利时和荷兰药剂师监管：为了公共利益还是私人利益？OAAI
OAAI分享收藏分享收藏The importance of interfaces in protein drug delivery - why is protein adsorption of interest in pharmaceutical formulations?界面在蛋白质药物递送中的重要性-为什么蛋白质吸附在药物制剂中是令人感兴趣的？
PREAI
分享收藏
查看更多内容