.png){kind=icon}
返回
Integrated optical device for Structured Illumination Microscopy
.png){kind=icon}
OA
AI
DOI：10.1364/OE.466225.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Structured Illumination Microscopy (SIM) is a key technology for high resolution and super-resolution imaging of biological cells and molecules. The spread of portable and easy-to-align SIM systems requires the development of novel methods to generate a light pattern and to shift it across the field of view of the microscope. Here we show a miniaturized chip that incorporates optical waveguides, splitters, and phase shifters, to generate a 2D structured illumination pattern suitable for SIM microscopy. The chip creates three point-sources, coherent and controlled in phase, without the need for further alignment. Placed in the pupil of a microscope's objective, the three sources generate a hexagonal illumination pattern on the sample, which is spatially translated thanks to thermal phase shifters. We validate and use the chip, upgrading a commercial inverted fluorescence microscope to a SIM setup and we image biological sample slides, extending the resolution of the microscope. (C) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
Keyword:
SPEED
LIGHT
GLASS
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
机构
引用论文
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution非线性结构照明显微镜: 理论上无限分辨率的宽场荧光成像OAAI
OAAI分享收藏GPU-accelerated real-time reconstruction in Python of three-dimensional datasets from structured illumination microscopy with hexagonal patterns基于GPU加速的六边形结构照明显微镜三维数据集的Python实时重建.png)
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCESOAAI
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCESIF3.7
OAAI分享收藏Thermal Phase Shifters for Femtosecond Laser Written Photonic Integrated Circuits用于飞秒激光写入光子集成电路的热移相器
OAAI
OAAI分享收藏SIMPLE: Structured illumination based point localization estimator with enhanced precision简单: 具有增强精度的基于结构化照明的点定位估计器OPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏Strategies for improved temporal response of glass-based optical switchesSCIENTIFIC REPORTSOAAI
SCIENTIFIC REPORTSIF3.9
OAAI分享收藏
查看更多内容