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Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling
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DOI：10.1021/acs.analchem.9b01876.png)
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摘要
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A quantitative description of glyco-alteration/differences in diseases can lead to the development of a diagnostic agent for use in vitro to monitor the degree of change in target glycoproteins. Analytical systems have been developed along with the progress of omits-oriented technologies. For clinical implementation, their full automation is required with an apparatus that is simple to operate. Here, we report an automatic analysis system for quantitative characterization of glyco-alteration/differences that depends on the unique strategy of bead arrays in a single tip. The alternative lectin array can obtain a minimum characterization of the glycan profile for nanogram quantities of an endogenous glycoprotein. A simple autopipetting robot produces the precise chemiluminescence detection of glycan-lectin interactions with a wide dynamic range that is superior to fluorescence-based lectin arrays. The tip-based array format enables automatic glycan profiling from sample pretreatment to detection with low variation and linear detection, which may facilitate the use of this lectin array in clinical practice.
Keyword:
LIVER FIBROSIS
MICROARRAY
GLYCOSYLATION
HEPA
STRATEGY
MARKER
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期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
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