Lineage conversion methodologies meet the reprogramming toolbox

Ignacio Sancho-Martinez

doi:10.1038/ncb2567

2012-09-03

101

PRE

Ignacio Sancho-Martinez *

Sung Hee Baek

Juan Carlos Izpisúa Belmonte

DOI：10.1038/ncb2567

原文链接

摘要

En 中文

Lineage conversion has recently attracted increasing attention as a potential alternative to the directed differentiation of pluripotent cells to obtain cells of a given lineage. Different means allowing for cell identity switch have been reported. Lineage conversion relied initially on the discovery of specific transcription factors generally enriched and characteristic of the target cell, and their forced expression in cells of a different fate. This approach has been successful in various cases, from cells of the hematopoietic systems to neurons and cardiomyocytes. Furthermore, recent reports have suggested the possibility of establishing a general lineage conversion approach bypassing pluripotency. This requires a first phase of epigenetic erasure achieved by short overexpression of the factors used to reprogram cells to a pluripotent state (such as a combination of Sox2, Klf4, c-Myc and Oct4), followed by exposure to specific developmental cues. Here we present these different direct conversion methodologies and discuss their potential as alternatives to using induced pluripotent stem cells and differentiation protocols to generate cell populations of a given fate.

Keyword:

PLURIPOTENT STEM-CELLS

ADULT HUMAN FIBROBLASTS

DEFINED TRANSCRIPTION FACTORS

TISSUE-SPECIFIC ENHANCERS

FUNCTIONAL-NEURONS

MOUSE FIBROBLASTS

DOPAMINERGIC-NEURONS

CARDIAC FIBROBLASTS

SOMATIC NUCLEI

IPS CELLS

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对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Nature Cell Biology

IF:

19.1

论文数:

6.2K

被引数:

4.8W

机构

salk institute

学者数:

3.2K

论文数: 2.2K

被引数: 8

seoul national university (snu)

学者数:

7.2W

论文数: 6.6W

被引数: 86

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