.png){kind=icon}
返回
Longitudinal zonation pattern in Arabidopsis root tip defined by a multiple structural change algorithm
.png){kind=icon}
OA
AI
DOI：10.1093/aob/mcw101.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Background and Aims The Arabidopsis thaliana root is a key experimental system in developmental biology. Despite its importance, we are still lacking an objective and broadly applicable approach for identification of number and position of developmental domains or zones along the longitudinal axis of the root apex or boundaries between them, which is essential for understanding the mechanisms underlying cell proliferation, elongation and differentiation dynamics during root development. Methods We used a statistics approach, the multiple structural change algorithm (MSC), for estimating the number and position of developmental transitions in the growing portion of the root apex. Once the positions of the transitions between domains and zones were determined, linear models were used to estimate the critical size of dividing cells (L-critD) and other parameters. Key Results The MSC approach enabled identification of three discrete regions in the growing parts of the root that correspond to the proliferation domain (PD), the transition domain (TD) and the elongation zone (EZ). Simultaneous application of the MSC approach and G2-to-M transition (CycB1;1DB:GFP) and endoreduplication (pCCS52A1:GUS) molecular markers confirmed the presence and position of the TD. We also found that the MADS-box gene XAANTAL1 (XAL1) is required for the wild-type (wt) PD increase in length during the first 2 weeks of growth. Contrary to wt, in the xal1 loss-of-function mutant the increase and acceleration of root growth were not detected. We also found alterations in L-critD in xal1 compared with wt, which was associated with longer cell cycle duration in the mutant. Conclusions The MSC approach is a useful, objective and versatile tool for identification of the PD, TD and EZ and boundaries between them in the root apices and can be used for the phenotyping of different genetic backgrounds, experimental treatments or developmental changes within a genotype. The tool is publicly available at www.ibiologia.com.mx/MSC_analysis.
Keyword:
Arabidopsis thaliana
cell differentiation
cell proliferation
proliferation domain
transition domain
elongation zone
root apical meristem
longitudinal zonation pattern
critical size of dividing cells
XAANTAL1
multiple structural change model
breakpoints
期刊
IF:
3.6
论文数:
6.9K
被引数:
2.4W
机构
引用论文
Cytokinins Control Endocycle Onset by Promoting the Expression of an APC/C Activator in Arabidopsis Roots.png)
CURRENT BIOLOGY
OAAI
CURRENT BIOLOGYIF7.5
OAAI分享收藏Estimating and testing linear models with multiple structural changes估计和测试具有多个结构变化的线性模型ECONOMETRICAOAAI
ECONOMETRICAIF7.1
OAAI分享收藏Spatio-temporal analysis of mitotic activity with a labile cyclin-GUS fusion proteinPLANT JOURNALOAAI
PLANT JOURNALIF5.7
OAAI分享收藏Histochemical analysis of root meristem activity in Arabidopsis thaliana using a cyclin:GUS (β-glucuronidase) marker linePLANT AND SOIL
PLANT AND SOILIF4.1
PREAI
分享收藏Determinate primary-root growth in seedlings of Sonoran Desert Cactaceae; its organization, cellular basis, and ecological significancePLANTA
PLANTAIF3.8
PREAI
分享收藏A Framework for Modeling, Simulation and Automatic Code Generation of Sensor Network Application
PREAI
分享收藏分享收藏Arabidopsis pdr2 reveals a phosphate-sensitive checkpoint in root development拟南芥pdr2揭示了根发育中的磷酸盐敏感检查点PLANT JOURNAL
PLANT JOURNALIF5.7
PREAI
分享收藏
查看更多内容