Low biomass bacterial transcriptomics takes shape

doi:10.1016/j.tim.2025.06.015

2025-07-07

PRE

Qinnan Yang

Eric C. Martens *

DOI：10.1016/j.tim.2025.06.015

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摘要

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Bornet等人应用了一种低输入细菌RNA-seq流程，对通过流式细胞分选（FACS）分离的人类肠道共生菌Bacteroides thetaiotaomicron的小、中、大细胞群体进行了转录组分析。他们的方法为测量遗传上相同但形态或其他FACS可分选特征（如抗体染色）不同的单个细菌细胞的转录变化开辟了途径。

Keyword:

Bacterial RNA-seq

Fluorescence activated cell sorting

Transcriptional profiling

Bacteroides thetaiotaomicron

Single-cell analysis

期刊

Trends in Microbiology

IF:

14.9

论文数:

3.9K

被引数:

1.9W

机构

university of michigan medical school

学者数:

598

论文数: 245

被引数: 0

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