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Method Development for Fecal Lipidomics Profiling
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DOI：10.1021/ac303011k.png)
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Abstract
En 中文
Robust methodologies for the analysis of fecal material will facilitate the understanding of gut (patho)-physiology and its role in health and disease and will help improve care for individual patients, especially high risk populations, such as premature infants Because lipidomics offers a biologically and analytically attractive approach, we developed a simple, sensitive, and quantitatively precise. method for profiling intact lipids in fecal material. The method utilizes two separate, complementary extraction chemistries, dichloromethane (DCM) and a methyl tert-butyl ether/hexafluoroisopropanol (MTBE) mixture, alone or with high pressure cycling. Extracts were assessed by liquid chromatography high-resolution mass spectrometry-based profiling with all ion higher energy collisional dissociation fragmentation in both positive and negative ionization modes. This approach provides both class-specific and lipid-specific fragments, enhancing lipid characterization. Solvents preferentially extracted lipids based on hydrophobicity. More polar species preferred MTBE; more hydrophobic compounds preferred DCM. Pressure cycling differentially increased the yield of some lipids. The platform enabled analysis of >500 intact lipophilic species with over 300 lipids spanning 6 LIPID MAPS categories identified in the fecal matter from premature infants. No previous report exists that provides these data; thus, this study represents a new paradigm for assessing nutritional health, inflammation, and infectious disease in vulnerable populations.
Keywords:
LIQUID-CHROMATOGRAPHIC ANALYSIS
QUANTITATIVE ISOLATION
EXTRACTION
OBESITY
GAS
ACIDS
SERUM
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