Microarray applications in neuroscience

Zhengyuan Luo

doi:10.1006/nbdi.2001.0387

2001-04-01

PRE

骆政园 (Zhengyuan Luo) *

Daniel H. Geschwind

DOI：10.1006/nbdi.2001.0387

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摘要

En 中文

Advances in all facets of technology from molecular biology to imaging and computational biology offer unprecedented opportunities for improving our understanding of the brain in health and disease. Oligonucleotide and cDNA microarray analysis, using a variety of DNA chips, is a recently developed high-throughput technique that allows for tour-de-force analysis of gene expression. We review this powerful technique, developed in genetics laboratories, with reference to applications in neurologic diseases in humans and the use of animal models. The typical microarray experiment is multistaged and includes preparation or purchase of arrays, preparation of target DNA and probe, target DNA hybridization, microarray scanning, and image analysis. The power and pitfalls of this technology are discussed in the context of neuroscience paradigms. Since unprecedented amounts of data are produced from microarray experiments, bioinformatics and modeling expertise are increasingly becoming critical components of this approach. (C) 2001 Academic Press.

Keyword:

DENSITY OLIGONUCLEOTIDE ARRAYS

GENE-EXPRESSION

CDNA MICROARRAY

MESSENGER-RNA

HOMEOBOX GENE

DIFFERENTIAL DISPLAY

MULTIPLE-SCLEROSIS

DNA MICROARRAY

MOUSE

BRAIN

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期刊

Neurobiology of Disease

IF:

5.6

论文数:

6.9K

被引数:

2.1W

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