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Microbial community interactions on a chip
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DOI：10.1073/pnas.2403510121.png)
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摘要
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Multispecies microbial communities drive most ecosystems on Earth. Chemical and biological interactions within these communities can affect the survival of individual members and the entire community. However, the prohibitively high number of possible interactions within a microbial community has made the characterization of factors that influence community development challenging. Here, we report a Microbial Community Interaction (mu CI) device to advance the systematic study of chemical and biological interactions within a microbial community. The mu CI creates a combinatorial landscape made up of an array of triangular wells interconnected with circular wells, which each contains either a different chemical or microbial strain, generating chemical gradients protein provided the target readout in the triangular wells, and antibiotics or microorthat gentamicin and vancomycin are antagonistic to each other in inhibiting the target B. cereus UW85, displaying weaker inhibitory activity when used in combination than alone. We identified three- member communities constructed with isolates from the plant both strain- level and community- level insight. The scalable geometric design of the influence microorganisms in solitary or community life.
Keyword:
microbial communities
microbial interactions
|microfluidics
fabricated microbial ecosystems
antibiotics
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IF:
9.1
论文数:
10.8W
被引数:
73.5W
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