.png){kind=icon}
返回
MicroBubble activated acoustic cell sorting
.png){kind=icon}
OA
AI
DOI：10.1007/s10544-017-0157-4.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave. In acoustophoresis, cells being positive acoustic contrast particles migrate to pressure nodes. On the contrary, air-filled polymer-shelled microbubbles being strong negative acoustic contrast particles migrate to pressure antinodes and can be used to selectively migrate target cells. As a proof of principle, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency. Moreover, 100% of the microbubble-cell conjugates migrated to the anti-node. Hence a better upstream affinity-capture has the potential to provide higher sorting efficiency. The BAACS technique expands the acoustic cell manipulation possibilities and offers cell-sorting solutions suited for applications at point of care.
Keyword:
Cell sorting
Acoustophoresis
Microbubble
Contrast agent
Microfluidic separation
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.3
论文数:
2.1K
被引数:
3.4K
机构
引用论文
Stable polymeric microballoons as multifunctional device for biomedical uses: Synthesis and characterization.png)
LANGMUIR
LANGMUIRIF3.9
PREAI
分享收藏分享收藏Elastomeric Negative Acoustic Contrast Particles for Affinity Capture AssaysANALYTICAL CHEMISTRYOAAI
ANALYTICAL CHEMISTRYIF6.7
OAAI分享收藏分享收藏On-chip free-flow magnetophoresis: Continuous flow separation of magnetic particles and agglomeratesANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏分享收藏
查看更多内容