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Microchip flow cytometry using electrokinetic focusing
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DOI：10.1021/ac990372u.png)
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摘要
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Flow cytometry of fluorescently labeled and unlabeled latex particles is demonstrated on a microfabricated device. The latex particles were detected and counted using laser light scattering and fluorescence coincidence measurements. Sample confinement was accomplished using electrokinetic focusing at a cross intersection, and detection occurred 50 mu m downstream from the intersection. Particles with diameters of 1 and 2 mu m were analyzed and distinguished from each other based on their light scattering intensity and fluorescence. A maximum sample throughput of 34 particles/s was achieved, Sample mixtures with varying proportions of fluorescently labeled and unlabeled particles were also analyzed and found to be within experimental error of the expected ratios.
Keyword:
OPEN-CHANNEL ELECTROCHROMATOGRAPHY
CAPILLARY ELECTROPHORESIS
POSTCOLUMN REACTOR
SAMPLE INJECTION
GLASS CHIPS
SEPARATIONS
DEVICE
CHROMATOGRAPHY
SYSTEMS
CELLS
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期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
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