.png){kind=icon}
返回
Microfluidic devices for cell based high throughput screening
.png){kind=icon}
OA
AI
DOI：10.1039/b918291h.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Cell based screening assays are increasingly used in drug discovery due to the physiological significance of the results and high content information obtained from them. Miniaturization of this format, currently carried out in microwell plates, is at its limit due to increased unnatural interaction of cells with walls inside micro-wells. In order to overcome this limitation, we present a new format for dynamically controlled, precise, spatial and temporal dosing of a continuous cell culture layer, using microfluidics. The device consists of a micropatterned nanoporous membrane layer that allows specific spatial locations in the continuous gel layer above, to be chemically addressed by external electric field through a microfluidic network below it. We demonstrate that the control of electric field across the nanoporous membrane leads to extremely precise dosing (similar to 50 mu g accuracy). Spot sizes of 200 mu m to 6 mm in diameter and inter-spot distances of 0.4-10 mm have been obtained. Microarray spot densities of 156 spots/cm(2) were obtained, which is five times higher than the densities used in current cell based assays. The capability of this method in handling small molecules, proteins and drugs is also demonstrated. This format of spatial dosing of continuous cell culture will enable further miniaturization of cell based assays and aid in high-throughput high-content screening.
Keyword:
DRUG DISCOVERY
CULTURE
CHANNELS
期刊
L
IF:
5.4
论文数:
9.0K
被引数:
3.3W
机构
引用论文
Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings在药物发现和开发环境中估算溶解度和渗透性的实验和计算方法
PREAI
分享收藏MFO-SFR: An Enhanced Moth-Flame Optimization Algorithm Using an Effective Stagnation Finding and Replacing Strategy.png)
MathematicsOAAI
MathematicsIF0
OAAI分享收藏The combination of nicotinamide mononucleotide and lycopene prevents cognitive impairment and attenuates oxidative damage in D-galactose induced aging models via Keap1-Nrf2 signalingGene
GeneIF0
PREAI
分享收藏
查看更多内容