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Misfolded opsin mutants display elevated β-sheet structure
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DOI：10.1016/j.febslet.2015.08.042.png)
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摘要
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Mutations in rhodopsin can cause misfolding and aggregation of the receptor, which leads to retinitis pigmentosa, a progressive retinal degenerative disease. The structure adopted by misfolded opsin mutants and the associated cell toxicity is poorly understood. Forster resonance energy transfer (FRET) and Fourier transform infrared (FTIR) microspectroscopy were utilized to probe within cells the structures formed by G188R and P23H opsins, which are misfolding mutants that cause autosomal dominant retinitis pigmentosa. Both mutants formed aggregates in the endoplasmic reticulum and exhibited altered secondary structure with elevated p-sheet and reduced a-helical content. The newly formed p-sheet structure may facilitate the aggregation of misfolded opsin mutants. The effects observed for the mutants were unrelated to retention of opsin molecules in the endoplasmic reticulum itself. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Keyword:
G protein-coupled receptor
Membrane protein
Protein aggregation
Protein misfolding
Secondary structure
Retinal degeneration
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3
论文数:
2.3W
被引数:
3.8W
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