Modeling human embryo adhesion using a microfluidic platform

Sofía Zaragozano

doi:10.1126/sciadv.adz2249

2026-07-10

Javier Moncayo-Arlandi

Javier González-Fernández

DOI：10.1126/sciadv.adz2249

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摘要

En 中文

胚胎黏附是着床的关键步骤，然而对这一过程的理解因缺乏能够复制子宫内膜生理状态的人体体外平台而受到阻碍。本文介绍了一种双通道微流控平台，该平台包含器官样细胞来源的子宫内膜上皮细胞和原代基质细胞。我们的模型再现了子宫内膜的重要特征，包括上皮极化、基质蜕膜化、细胞外囊泡释放以及激素诱导的容受性。我们使用小鼠胚胎和人囊胚对该模型进行了验证，并发现胚胎表现出初始黏附的特征。这些特征包括通过极化的滋养外胚层建立胚胎-上皮接触、内细胞团重新定位以及谱系重组。此外，人胚胎分泌βhCG，表明滋养层具有功能。因此，本研究提供了一个平台，用于研究胚胎黏附和子宫内膜容受性的关键特征以及影响胚胎-子宫内膜相互作用的功能障碍。

期刊

Science Advances

IF:

12.5

论文数:

2.1W

被引数:

18.1W

机构

clínica el pilar

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论文数: 1

被引数: 0

incliva biomedical research institute

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被引数: 1

next fertility

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论文数: 1

被引数: 0

University of Pennsylvania

学者数:

1.2W

论文数: 4.4K

被引数: 11.8W

carlos simon foundation

学者数:

论文数: 4

被引数: 0

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