.png){kind=icon}
返回
Modulated pattern scanning microscopy
.png){kind=icon}
PRE
AI
DOI：10.1364/OL.451958.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
In confocal microscopy, the effective optical transfer function (OTFeff) with Gaussian plane wave illumination covers very few high-frequency components, which prohibits further improvement of the resolution. We propose modulated pattern scanning microscopy (MPSM) to achieve super-resolution imaging. In MPSM, the phase of the illumination beam is modulated to reassign the OTFeff in the Fourier domain. The phase mask is designed using an optimization algorithm to obtain the fluorescence emission pattern with rich high-frequency components. Then, the postprocessing algorithms are adapted to retrieve the super-resolved images from the modulated recordings. Simulation and experiment demonstrate that MPSM increases the resolution approximately 1.3 times better than confocal microscopy. Compared with conventional deconvolution, MPSM exhibits a higher signal-to-noise ratio. (C) 2022 Optica Publishing Group
Keyword:
LATERAL RESOLUTION
ILLUMINATION
CONTRAST
DEPTH
LIMIT
BEAM
期刊
IF:
3.3
论文数:
4.0W
被引数:
7.6W
机构
引用论文
Model estimates of CO2 emissions from soil in response to global warming响应全球变暖的土壤CO2排放模型估计.png)
Nature
NatureIF0
PREAI
分享收藏Breaking the Diffraction Barrier Using Fluorescence Emission Difference MicroscopySCIENTIFIC REPORTS
OAAI
SCIENTIFIC REPORTSIF3.9
OAAI分享收藏Confocal laser scanning microscopy with spatiotemporal structured illuminationOPTICS LETTERS
OPTICS LETTERSIF3.3
PREAI
分享收藏Intensity Weighted Subtraction Microscopy Approach for Image Contrast and Resolution Enhancement增强图像对比度和分辨率的强度加权减影显微方法SCIENTIFIC REPORTSOAAI
SCIENTIFIC REPORTSIF3.9
OAAI分享收藏Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)OPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏
查看更多内容