.png){kind=icon}
返回
Monitoring global messenger RNA changes in externally controlled microarray experiments
.png){kind=icon}
OA
AI
DOI：10.1038/sj.embor.embor798.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Expression profiling is a universal tool, with a range of applications that benefit from the accurate determination of differential gene expression. To allow normalization using endogenous transcript levels, current microarray analyses assume that relatively few transcripts vary, or that any changes that occur are balanced. When normalization using endogenous genes is carried out, changes in expression levels are calculated relative to the behaviour of most of the transcripts. This does not reflect absolute changes if global shifts in messenger RNA populations occur. Using external RNA controls, we have set up microarray experiments to monitor global changes. The levels of most mRNAs were found to change during yeast stationary phase and human heat shock when external controls were included. Even small global changes had a significant effect on the number of genes reported as being differentially expressed. This suggests that global mRNA changes occur more frequently than is assumed at present, and shows that monitoring such effects may be important for the accurate determination of changes in gene expression.
Keyword:
EXPRESSION
NORMALIZATION
STANDARDS
DESIGN
ISSUES
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
E
IF:
6.2
论文数:
6.4K
被引数:
2.1W
机构
暂无机构信息
引用论文
Etanercept plus methylprednisolone as initial therapy for acute graft-versus-host disease.png)
BloodOAAI
BloodIF0
OAAI分享收藏Control genes and variability: Absence of ubiquitous reference transcripts in diverse mammalian expression studiesGENOME RESEARCH
OAAI
GENOME RESEARCHIF5.5
OAAI分享收藏Issues in cDNA microarray analysis: quality filtering, channel normalization, models of variations and assessment of gene effectsNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏Ranking:: a closer look on globalisation methods for normalisation of gene expression arrays -: art. no. e50NUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variationNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏
查看更多内容