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Mouse Embryos as In Vivo Models for Proteomic Identification and Functional Analysis of Missing Proteins
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DOI：10.1016/j.ab.2026.116138.png)
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Abstract
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• Establishment of the Mouse Embryo Platform: The research confirmed that the mouse embryo provides a useful and feasible platform to detect and further understand the expression pattern and subsequent physiological functions of human missing proteins. Utilizing mass spectrometric analysis and newly developed software, the study successfully identified 112 missing proteins in the mouse embryonic samples. • Development of New Alignment Software: The study developed and applied a new alignment software (available at https://bio.vexp.idv.tw/∼tjm/fu_match/ ) to compare peptides identified via mass spectrometry from mouse embryos with putative peptides listed in the newly established human missing protein database. • Demonstration of Temporary Protein Functionality: The missing protein NKX1 exhibited a temporary burst expression (showing an 8-fold increase) from 7 to 7.5 days post conception (dpc), and then dramatically diminished at 8 dpc and in various adult organs. Furthermore, the signaling pathways predicted for several identified missing proteins (NKX1, PTCHD1, ASXL3, and OTOGL) were found to be involved in crucial processes such as neural development, inflammation, mitochondrial function, and anti-aging
Keywords:
Mouse embryo
Missing proteins
Proteomic identification
Functional analysis
Mass spectrometry
Journal
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2.5
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272
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3.3W
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