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Multiple pathways guide oxygen diffusion into flavoenzyme active sites
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DOI：10.1073/pnas.0903809106.png)
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摘要
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Dioxygen (O-2) and other gas molecules have a fundamental role in a variety of enzymatic reactions. However, it is only poorly understood which O-2 uptake mechanism enzymes employ to promote efficient catalysis and how general this is. We investigated O-2 diffusion pathways into monooxygenase and oxidase flavoenzymes, using an integrated computational and experimental approach. Enhanced-statistics molecular dynamics simulations reveal spontaneous protein-guided O-2 diffusion from the bulk solvent to preorganized protein cavities. The predicted protein-guided diffusion paths and the importance of key cavity residues for oxygen diffusion were verified by combining site-directed mutagenesis, rapid kinetics experiments, and high-resolution X-ray structures. This study indicates that monooxygenase and oxidase flavoenzymes employ multiple funnel-shaped diffusion pathways to absorb O-2 from the solvent and direct it to the reacting C4a atom of the flavin cofactor. The difference in O-2 reactivity among dehydrogenases, monooxygenases, and oxidases ultimately resides in the fine modulation of the local environment embedding the reactive locus of the flavin.
Keyword:
computational biochemistry
enzymology
flavin
oxygen reactivity
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9.1
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10.8W
被引数:
73.5W
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