.png){kind=icon}
返回
Multiplex cell microarrays for high-throughput screening
.png){kind=icon}
PRE
AI
DOI：10.1039/c6lc00831c.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Microarray technology was developed in the early 1990s to measure the transcription levels of thousands of genes in parallel. The basic premise of high-density arraying has since been expanded to create cell microarrays. Cells on chip are powerful experimental tools for high-throughput and multiplex screening of samples or cellular functions. Miniaturization increases assay throughput while reducing both reagent consumption and cell population heterogeneity effect, making these systems attractive for a wide range of assays, from drug discovery to toxicology, stem cell research and therapy. It is usual to functionalize the surface of a substrate to design cell microarrays. One form of cell microarrays, the transfected cell microarray, wherein plasmid DNA or siRNA spotted on the surface of a substrate is reverse-transfected locally into adherent cells, has become a standard tool for parallel cell-based analysis. With the advent of technology, cells can also be directly spotted onto functionalized surfaces using robotic fluid-dispensing devices or printed directly on bio-ink material. We are providing herein an overview of the latest developments in optical cell microarrays allowing high-throughput and high-content analysis.
Keyword:
SECRETED ALKALINE-PHOSPHATASE
GENE-EXPRESSION
ELECTROCHEMICAL DETECTION
PLASMA POLYMERIZATION
ON-CHIP
SURFACE FUNCTIONALIZATION
CYTOMETRY PLATFORM
SINGLE CELLS
STEM
PROTEIN
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
L
IF:
5.4
论文数:
9.0K
被引数:
3.3W
机构
引用论文
分享收藏分享收藏High-Throughput Screening (HTS) of Anticancer Drug Efficacy on a Micropillar/Microwell Chip Platform.png)
ANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine基于液滴的微流控平台用于IL-10细胞因子的单个T细胞分泌分析
OAAI
OAAI分享收藏Single cells and intracellular processes studied by a plasmonic-based electrochemical impedance microscopyNATURE CHEMISTRY
OAAI
NATURE CHEMISTRYIF20.2
OAAI分享收藏分享收藏分享收藏
查看更多内容