Multisite Gateway Calculations: Excel spreadsheet v1

Multisite Gateway cloning provides a modular tool to assemble plasmid vectors suitable for numerous applications. The assembly of Multisite Gateway plasmids requires the correct ratios of three entry vectors and the destination backbone to favor correct recombination by Gateway LR Clonase II Plus. While simple, the calculation of recombination mixes is a reoccurring task when using Multisite Gateway that benefits from automation and proper documentation. This protocol provides a simple Excel-based spreadsheet to simplify the involved calculations based on providing individual vector lengths and working stock concentrations. Using this Multisite Gateway calculations spreadsheet supports correct reaction mix assembly, scaling of cloning reactions, cloning documentation, and troubleshooting.