.png){kind=icon}
返回
Mycobacteria Exploit Host Hyaluronan for Efficient Extracellular Replication
.png){kind=icon}
OA
AI
DOI：10.1371/journal.ppat.1000643.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
In spite of the importance of hyaluronan in host protection against infectious organisms in the alveolar spaces, its role in mycobacterial infection is unknown. In a previous study, we found that mycobacteria interact with hyaluronan on lung epithelial cells. Here, we have analyzed the role of hyaluronan after mycobacterial infection was established and found that pathogenic mycobacteria can grow by utilizing hyaluronan as a carbon source. Both mouse and human possess 3 kinds of hyaluronan synthases (HAS), designated HAS1, HAS2, and HAS3. Utilizing individual HAS-transfected cells, we show that HAS1 and HAS3 but not HAS2 support growth of mycobacteria. We found that the major hyaluronan synthase expressed in the lung is HAS1, and that its expression was increased after infection with Mycobacterium tuberculosis. Histochemical analysis demonstrated that hyaluronan profoundly accumulated in the granulomatous legion of the lungs in M. tuberculosis-infected mice and rhesus monkeys that died from tuberculosis. We detected hyaluronidase activity in the lysate of mycobacteria and showed that it was critical for hyaluronan-dependent extracellular growth. Finally, we showed that L-Ascorbic acid 6-hexadecanoate, a hyaluronidase inhibitor, suppressed growth of mycobacteria in vivo. Taken together, our data show that pathogenic mycobacteria exploit an intrinsic host-protective molecule, hyaluronan, to grow in the respiratory tract and demonstrate the potential usefulness of hyaluronidase inhibitors against mycobacterial diseases.
Keyword:
HEPARIN-BINDING HEMAGGLUTININ
DENDRITIC CELLS
TUBERCULOSIS
LUNG
OLIGOSACCHARIDES
INFLAMMATION
INHIBITION
SYNTHASES
CD44
IDENTIFICATION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
4.9
论文数:
1.1W
被引数:
5.2W
机构
引用论文
分享收藏Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids
PREAI
分享收藏Oligosaccharides of hyaluronan activate dendritic cells via toll-like receptor 4透明质酸寡糖通过toll样受体4激活树突状细胞OAAI
OAAI分享收藏Persistence of DNA from Mycobacterium tuberculosis in superficially normal lung tissue during latent infection.png)
LANCET
LANCETIF88.5
PREAI
分享收藏
查看更多内容