Nanoparticles Enhanced Self-Driven Microfludic Biosensor

Chunxiu Liu

doi:10.3390/mi11040350

Nanoparticles Enhanced Self-Driven Microfludic Biosensor

2020-03-27

10

OA

AI

DOI：10.3390/mi11040350

原文链接

查看原文

摘要

En 中文

C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods.

Keyword:

microfluidic biosensor

amplification effect of nanoparticle

optomagnetic detection

sandwich assay

Nanoparticles Enhanced Self-Driven Microfludic Biosensor

摘要

AI总结

期刊

机构