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New technologies for high-throughput screening
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DOI：10.1016/S1367-5931(97)80111-1.png)
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摘要
En 中文
To screen efficiently the millions of compounds that are synthesized using combinatorial and automated methods, dramatically improved assay technologies are currently needed. In 96-well microtiter plates, nonradioactive techniques (primarily fluorimetric) and cell-based functional methods have moved to the cutting edge, while clever assays that extract information from large bead-based combinatorial libraries have begun to show considerable promise. In the future, miniaturized assays that break out of the 96-well format will be enabled by innovative technologies for high-throughput screening.
Keyword:
FLUORESCENCE ENERGY-TRANSFER
SENSITIVE DETECTION
PROTEIN
BINDING
POLARIZATION
CHEMILUMINESCENCE
BIOTECHNOLOGY
IMMUNOASSAY
ANTIBODIES
DOMAIN
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