.png){kind=icon}
返回
Noise reduction by upstream open reading frames
.png){kind=icon}
OA
AI
DOI：10.1038/s41477-022-01136-8.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Gene expression is prone to burst production, making it a highly noisy process that requires additional controls. Upstream open reading frames (uORFs) are widely present in the 5' leader sequences of 30-50% of eukaryotic messenger RNAs1-3. The translation of uORFs can repress the translation efficiency of the downstream main coding sequences. Whether the low translation efficiency leads to a different variation, or noise, in gene expression has not been investigated, nor has the direct biological impact of uORF-repressed translation. Here we show that uORFs achieve low but precise protein production in plant cells, possibly by reducing the protein production rate. We also demonstrate that, by buffering a stable TIMING OF CAB EXPRESSION 1 (TOC1) protein production level, uORFs contribute to the robust operation of the plant circadian clock. Our results provide both an action model and the biological impact of uORFs in translational control to mitigate transcriptional noise for precise protein production.
Keyword:
PROTEIN EXPRESSION
GENE-EXPRESSION
TRANSLATION
RIBOSOME
DEGRADATION
REVEALS
TOC1
STOCHASTICITY
CLOCKS
CELLS
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
13.6
论文数:
2.8K
被引数:
1.8W
机构
引用论文
Temporal changes in plasma levels and metabolism of ketone bodies by liver and brain after ethanol and/or starvation in C57BL/6J miceC57BL/6J小鼠乙醇和/或饥饿后血浆水平和肝脏和大脑中酮体代谢的时间变化
PREAI
分享收藏Fast evaluation of fluctuations in biochemical networks with the linear noise approximation.png)
GENOME RESEARCHOAAI
GENOME RESEARCHIF5.5
OAAI分享收藏Contribution of RNA Degradation to Intrinsic and Extrinsic Noise in Gene ExpressionCELL REPORTSOAAI
CELL REPORTSIF6.9
OAAI分享收藏Arabidopsis mesophyll protoplasts:: a versatile cell system for transient gene expression analysis拟南芥叶肉原生质体:: 用于瞬时基因表达分析的通用细胞系统NATURE PROTOCOLSOAAI
NATURE PROTOCOLSIF16
OAAI分享收藏Antithetic Integral Feedback Ensures Robust Perfect Adaptation in Noisy Biomolecular NetworksCELL SYSTEMSOAAI
CELL SYSTEMSIF7.7
OAAI分享收藏
查看更多内容