.png){kind=icon}
返回
Noncanonical processing by animal Microprocessor
.png){kind=icon}
OA
AI
DOI：10.1016/j.molcel.2023.05.004.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Microprocessor (MP), DROSHA-DGCR8, processes primary miRNA transcripts (pri-miRNAs) to initiate miRNA biogenesis. The canonical cleavage mechanism of MP has been extensively investigated and comprehensively validated for two decades. However, this canonical mechanism cannot account for the pro-cessing of certain pri-miRNAs in animals. In this study, by conducting high-throughput pri-miRNA cleavage assays for approximately 260,000 pri-miRNA sequences, we discovered and comprehensively characterized a noncanonical cleavage mechanism of MP. This noncanonical mechanism does not need several RNA and protein elements essential for the canonical mechanism; instead, it utilizes previously unrecognized DROSHA dsRNA recognition sites (DRESs). Interestingly, the noncanonical mechanism is conserved across animals and plays a particularly significant role in C. elegans. Our established noncanonical mechanism elucidates MP cleavage in numerous RNA substrates unaccounted for by the canonical mechanism in animals. This study suggests a broader substrate repertoire of animal MPs and an expanded regulatory landscape for miRNA biogenesis.
Keyword:
PRIMARY MICRORNAS
MOLECULAR-BASIS
READ ALIGNMENT
CLEAVAGE
DROSHA
RECOGNITION
RNA
COMPLEX
STEM
IDENTIFICATION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
16.6
论文数:
1.0W
被引数:
8.6W
机构
暂无机构信息
引用论文
Reduced graphene oxide–CdS nanocomposites with enhanced visible-light photoactivity synthesized using ionic-liquid precursors使用离子液体前体合成的具有增强的可见光光活性的还原氧化石墨烯-CdS纳米复合材料.png)
New J. Chem.
New J. Chem.IF0
PREAI
分享收藏
分享收藏The internal loops in the lower stem of primary microRNA transcripts facilitate single cleavage of human MicroprocessorNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAI分享收藏分享收藏分享收藏
查看更多内容