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On-Chip Mirror Enhanced Multiphoton Upconversion Super-Resolution Microscopy
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DOI：10.1021/acs.nanolett.3c00763.png)
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Multiphoton upconversion super-resolution microscopy(MPUM) isa promising imaging modality, which can provide increased resolutionand penetration depth by using nonlinear near-infrared emission lightthrough the so-called transparent biological window. However, a highexcitation power is needed to achieve emission saturation, which increasesphototoxicity. Here, we present an approach to realize the nonlinearsaturation emission under a low excitation power by a simply designedon-chip mirror. The interference of the local electromagnetic fieldcan easily confine the point spread function to a specific area toincrease the excitation efficiency, which enables emission saturationunder a lower excitation power. With no additional complexity, themirror assists to decrease the excitation power by 10-fold and facilitiesthe achievement of a lateral resolution around 35 nm, 1/28th of theexcitation wavelength, in imaging of a single nanoparticle on-chip.This method offers a simple solution for super-resolution enhancementby a predesigned on-chip device.
Keyword:
upconversion nanoparticles
on-chip super-resolution
mirror enhanced
multiphoton imaging
stimulateddepletion microscopy
self-interference
期刊
IF:
9.1
论文数:
2.7W
被引数:
16.5W
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