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En 中文
Abstract
The past decade has witnessed an enormous growth in the application of optical microscopy for micron and submicron level investigations in a wide variety of disciplines. Rapid development of new fluorescent labels has accelerated the expansion of fluorescence microscopy in laboratory applications and research. Advances in digital imaging and analysis have also enabled microscopists to acquire quantitative measurements quickly and efficiently on specimens ranging from photosensitive caged compounds and synthetic ceramic superconductors to real‐time fluorescence microscopy of living cells in their natural environment. Optical microscopy, with help of digital video, can also be used to image very thin optical sections, and confocal optical systems are now in operation at most major research institutions.
This article discusses the history and current use of optical microscopy. Specific topics include microscope objectives, eye pieces, condensers, and optical aberrations. Recommendations are made. Contrast enhancing techniques, reflected light microscopy, darkfield microscopy, phase contrast microscopy, Rheinberg illumination, and differential interference contrast are detailed. Other topics include photomicrography both on film and with electronic analog and digital imaging systems and photographic films.
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