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Oxidative biotransformations using oxygenases

delete2002-04-01
delete143
PRE
AI
Z
Zhi Li
V
van Beilen, JB
W
Wouter A. Duetz
A
Andreas Schmid
A
Anna De Raadt
H
Herfried Griengl
B
Bernard Witholt
DOI：10.1016/S1367-5931(02)00296-Xdelete
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摘要

摘要

En 中文
Considerable progress has been made in manipulating oxidative biotransformations using oxygenases. Substrate acceptance, catalytic activity, regioselectivity and stereoselectivity have been improved significantly by substrate engineering, enzyme engineering or biocatalyst screening. Preparative biotransformations have been carried out to synthesize useful pharmaceutical intermediates or chiral synthons on the gram to several-hundred-gram scale, by use of whole cells of wild type or recombinant strains. The synthetic application of oxygenases in vitro has been shown to be possible by enzymatic or electrochemical regeneration of NADH or NADPH.
Keyword:
BAEYER-VILLIGER OXIDATIONS
CYCLOHEXANONE MONOOXYGENASE
ACINETOBACTER-CALCOACETICUS
ASYMMETRIC HYDROXYLATION
MICROBIAL HYDROXYLATION
DIRECTED EVOLUTION
WHOLE CELLS
LIPOXYGENASE
HYDROCARBONS
DIHYDROXYLATION
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Current Opinion in Chemical Biology 封面图
Current Opinion in Chemical Biology
IF:
6.1
论文数:
3.1K
被引数:
1.1W

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