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PDMS-based microfluidics for proteomic analysis
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DOI：10.1039/b606394b.png)
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摘要
En 中文
A microfluidic poly( dimethylsiloxane) (PDMS) microdevice was realized, combining on-line protein electrophoretic separation, selection, and digestion of a protein of interest for identification by mass spectrometry. The system includes eight integrated valves and one micropump dedicated to control the flow operations. Myoglobin was successfully isolated from bovine serum albumin (BSA), then selected using integrated valves and digested in a rotary micromixer. Proteolytic peptides were recovered from the micromixer for protein identification. Total analysis from sample injection to protein identification is performed under 30 minutes, with samples of tens of nanolitres. The paper shows that PDMS technology can be successfully used for integrating complex preparation protocols of proteic samples prior to MS analysis.
Keyword:
CAPILLARY-ELECTROPHORESIS SEPARATIONS
ELECTROSPRAY MASS-SPECTROMETRY
PROTEIN IDENTIFICATION
SURFACE MODIFICATION
DIGESTION
CHIPS
INTEGRATION
TECHNOLOGY
MICROCHIPS
PLASMA
期刊
A
IF:
3.3
论文数:
1.6W
被引数:
3.3W
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Integrated microanalytical technology enabling rapid and automated protein identification.png)
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