.png){kind=icon}
.png){kind=icon}
Return
Physicochemical and Antimicrobial Characterization of Nanobubbles Reveals Physical Disruption is the Primary Mode of Biofilm Inactivation
.png){kind=icon}
OA
AI
N
M
M
D
A
U
J
DOI：10.1021/acsestwater.6c00252.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Biofilm-associated contamination represents a persistent and costly challenge across environmental systems, causing reduced efficacy of disinfectants. Recently, nanobubbles (NBs) have shown promise for biofilm decontamination; yet, their underpinning mode of action remains a topic of debate. In this study, the interaction of air-generated NBs with Escherichia coli and Staphylococcus aureus biofilms was investigated. NBs were generated using a venturi nozzle and characterized using Nanoparticle Tracking Analysis, revealing a NB density of 5.66 × 108 particles/mL and a mean diameter of 84 nm. Application of NB solution to microbial biofilms resulted in a 2.16 log reduction for E. coli and 1.52 log reduction for S. aureus, along with visible morphological changes such as cell collapse, wrinkling, and matrix disruption. ESR spin trapping confirmed hydroxyl radical formation, but intracellular ROS and lipid peroxidation levels were minimal and, in some cases, not significantly different from Milli-Q water controls. After 28 days, NBs remained present and continued to demonstrate antimicrobial activity, biofilm disruption, and some ROS activity. These findings indicate that although hydroxyl radicals are generated, oxidative stress is not the dominant antimicrobial mechanism under the examined conditions, suggesting physical biofilm disruption is the primary mode of action.
Keywords:
Bacteria
Biofilms
Electron paramagnetic resonance spectroscopy
Hydroxyls
Nanobubbles
nanobubbles
ultrafine bubbles
reactive oxygen species
hydroxyl radicals
biofilms
decontamination
oxidative stress
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
A
IF:
4.3
Papers:
2.4K
Citations:
4.9K
Organization
Cited Papers