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Physiological and metabolic responses of cobia (Rachycentron canadum) juveniles fed fish oil-free diets with different LC-PUFA/C18 PUFA ratios

delete2026-05-05
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S
Sabrina Vitória Ferreira da Costa
B
Bruno Cavalheiro Araújo
R
Renata Guimarães Moreira
R
Renato Massaaki Honji
J
Júlia Sousa Siqueira
P
Paulo H. de Mello
A
Alexandre Wagner Silva Hilsdorf
M
Micheli Zaminhan‐Hassemer *
DOI:10.1016/j.anifeedsci.2026.116800delete
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Abstract

Abstract

En 中文
This study evaluated the replacement of fish oil (FO) with soybean oil (SO), combined with varying levels of long-chain polyunsaturated fatty acids (LC-PUFAs), in diets for cobia juveniles (Rachycentron canadum). Four isoenergetic and isoproteic diets were formulated: FO (control, 88 g kg⁻¹ FO), D1 (60 g kg⁻¹ SO), D2 (45 g kg⁻¹ SO), and D3 (30 g kg⁻¹ SO). All diets included 20 g kg⁻¹ coconut oil and increasing amounts of arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Growth performance was not significantly affected by dietary treatment. In general, saturated fatty acids decreased, while monounsaturated and polyunsaturated fatty acids increased in tissues of SO-fed groups compared with the FO. Muscle LC-PUFA contents reflected dietary levels, except for ARA in D3. Notably, DHA deposition was highest in D3, despite lower dietary DHA compared to the FO diet. Overall, the liver exhibited a fatty acid composition similar to that observed in the muscle. Histological analysis revealed larger hepatic lipid vacuoles in FO and D1, and smaller in D2 and D3. No significant differences were detected in the expression of lipid-related genes. These results suggest that SO, when supplemented with essential LC-PUFAs, may replace FO without negatively affecting growth or lipid metabolism, with higher LC-PUFA supplementation (D3) being associated with increased LC-PUFA deposition, particularly DHA, in cobia muscle.
Keywords:
Fish nutrition
FO replacement
lipid metabolism
fatty acid profile
LC-PUFA
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Journal

Animal Feed Science and Technology cover
Animal Feed Science and Technology
IF:
2.7
Papers:
473
Citations:
1.5W

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