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Polarization microscopy: from ensemble structural imaging to single-molecule 3D orientation and localization microscopy
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DOI：10.1364/OPTICA.502119.png)
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Abstract
En 中文
Optical contrasts in microscopy are sensitive to light polarization, whose interaction with molecular dipoles provides an important lever for probing molecular orientation. Polarization microscopy has evolved considerably during the last decade, integrating strategies ranging from traditional linear dichroism to single-molecule orientation and localization imaging. This review aims to provide a summary of concepts and techniques behind orientation and structural imaging at the molecular level, from ensemble microscopy in 2D to single-molecule super-resolution microscopy in 3D. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
Keywords:
POINT-SPREAD-FUNCTION
2ND-HARMONIC GENERATION MICROSCOPY
RESOLVED NONLINEAR MICROSCOPY
FLUORESCENCE MICROSCOPY
SUPERRESOLUTION MICROSCOPY
3-DIMENSIONAL ORIENTATION
CONFORMATIONAL-CHANGES
ROTATIONAL MOBILITY
DIPOLE ORIENTATION
DIFFRACTION-LIMIT
Journal
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8.5
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2.4K
Citations:
2.1W