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Principal component analysis-based quantitative differential interference contrast microscopy
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DOI：10.1364/OL.44.000045.png)
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Classical differential interference contrast (DIC) microscopy is an excellent tool for rendering high-contrast images to observe transparent specimen. Though the monochromatic shadow-cast image qualitatively reflects the specimen configuration, its phase can hardly be extracted, inevitably limiting its application in quantitative measurements. In order to extend its application, we propose a quantitative DIC (qDIC) microscopy technique, which can extract the specimen phase from phase-shifting beam-shearing interference images combining principal component analysis (PCA) and phase integration methods. Proved by both simulations and experiments, the PCA-based qDIC microscopy can rapidly and accurately retrieve the quantitative specimen phase. The new proposed technique shares with conventional DIC the advantage of high-contrast imaging, especially for transparent label-free specimens, but transforms DIC microscopy into a quantitative phase microscopy technique. We envision PCA-based qDIC microscopy as a future quantitative biological imaging modality. (C) 2018 Optical Society of America
Keyword:
DIC MICROSCOPY
PHASE
LIGHT
期刊
IF:
3.3
论文数:
4.0W
被引数:
7.6W
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Analysis of the principal component algorithm in phase-shifting interferometry.png)
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