Probing microbubble targeting with atomic force microscopy

doi:10.1016/j.colsurfb.2010.05.022

2010-10-01

PRE

DOI：10.1016/j.colsurfb.2010.05.022

原文链接

原文求助

摘要

En 中文

Microbubble science is expanding beyond ultrasound imaging applications to biological targeting and drug/gene delivery. The characteristics of molecular targeting should be tested by a measurement system that can assess targeting efficacy and strength. Atomic force microscopy (AFM) is capable of piconewton force resolution, and is reported to measure the strength of single hydrogen bonds. An in-house targeted microbubble modified using the biotin-avidin chemistry and the CD31 antibody was used to probe cultures of Sk-Hep1 hepatic endothelial cells. We report that the targeted microbubbles provide a single distribution of adhesion forces with a median of 93 pN. This interaction is assigned to the CD31 antibody-antigen unbinding event. Information on the distances between the interaction forces was obtained and could be important for future microbubble fabrication. In conclusion, the capability of single microbubbles to target cell lines was shown to be feasible with AFM. (c) 2010 Elsevier B.V. All rights reserved.

Keyword:

Targeted microbubbles

Atomic force microscope

Microbubble adhesion

Microbubble-cell avidity

CD31

PECAM

AI总结

对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。

期刊

Colloids and Surfaces B: Biointerfaces

IF:

5.6

论文数:

1.4W

被引数:

4.2W

机构

Royal Infirmary of Edinburgh

学者数:

3.9K

论文数: 3.0K

被引数: 3

University of Edinburgh

学者数:

5.2W

论文数: 4.6W

被引数: 71

引用论文

Luminescent Metal‐Phenolic Networks for Multicolor Particle Labeling

Angewandte Chemie

IF0

2021-10-18

PREAI

Zhixing Lin; Jiajing Zhou; Yijiao Qu; Shuaijun Pan; Yiyuan Han; René P. M. Lafleur; Jingqu Chen; Christina Cortez‐Jugo; Joseph J. Richardson; Frank Caruso

Effect of receptor-ligand affinity on the strength of endothelial cell adhesion

BIOPHYSICAL JOURNAL

IF3.1

1996-11-01

168