Profiling the epigenome using long-read sequencing

Tianyuan Liu

doi:10.1038/s41588-024-02038-5

2025-01-08

PRE

Tianyuan Liu

Ana Conesa

DOI：10.1038/s41588-024-02038-5

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摘要

En 中文

单分子长读长测序（LRS）技术的出现，由Oxford Nanopore Technologies和Pacific Biosciences开发，已经彻底改变了基因组学、转录组学以及最近表观基因组学的研究。这些技术提供了独特的优势，包括直接检测甲基化DNA以及同时评估跨越多个千碱基的DNA序列及其修饰，并在单分子水平上进行评估。这促进了分析染色质状态的新方法的开发，并使得整合DNA甲基化、染色质可及性、转录因子结合和组蛋白修饰的数据成为可能，从而促进了全面的表观基因组分析。由于近期取得的进展，可以使用LRS方法检测替代、新兴和翻译中的转录本。本文讨论了基于LRS的实验和计算策略，用于表征染色质状态，并强调了它们相对于短读长测序方法的优点。此外，我们展示了如何整合各种长读长方法来设计多组学研究，以探究染色质状态与转录动力学之间的关系。

Keyword:

RNA-POLYMERASE-II

DNA METHYLATION

SINGLE-MOLECULE

HI-C

GENOME

SEQ

CHALLENGES

LANDMARK

CHEC

期刊

Nature Genetics

IF:

论文数:

780

被引数:

241

机构

Spanish Natl Res Council

学者数:

论文数: 37

被引数: 17

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