Protein separation by sequential selective complex coacervation

doi:10.1016/j.jcis.2023.06.119

2023-11-01

PRE

Martien A. Cohen Stuart

王俊有 (Junyou Wang) *

DOI：10.1016/j.jcis.2023.06.119

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摘要

En 中文

In food manufacturing and particular biomedical products selected proteins are often required. Obtaining the desired proteins in a pure form from natural resources is therefore important, but often very challenging. Herein, we design a sequential coacervation process that allows to efficiently isolate and purify proteins with different isoelectric points (pIs) from a mixed solution, namely Bovine Serum Albumin (BSA, pI = 4.9) and Peroxidase from Horseradish (HRP, pI = 7.2). The key to separation is introducing a suitable polyelectrolyte that causes selective complex coacervation at appropriate pH and ionic strength. Specifically, polyethyleneimine (PEI), when added into the mixture at pH 6.0, produces a coacervation which exclusively contains BSA, leading to a supernatant solution containing 100 % HRP with a purity of 91 %. After separating the dilute and dense phases, BSA is recovered by adding poly(acrylic acid) (PAA) to the concentrated phase, which displaces BSA from the complex because it interacts more strongly with PEI. The supernatant phase after this step contains approximately 75 % of the initial amount of BSA with a purity of 99 %. Our results confirm that coacervation under well defined conditions can be selective, enabling separation of proteins with adequate purity. Therefore, the established approach demonstrates a facile , sustainable strategy with potential for protein separation at industrial scale.

Keyword:

Polyelectrolytes

Complex coacervation

Selective complexation

Protein separation

期刊

Journal of Colloid and Interface Science

IF:

9.7

论文数:

3.7W

被引数:

14.7W

机构

shanghai jiao tong university

学者数:

15.7W

论文数: 11.7W

被引数: 159

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