.png){kind=icon}
返回
Pulse-Picking Multimodal Nonlinear Optical Microscopy
.png){kind=icon}
PRE
AI
DOI：10.1021/acs.analchem.2c03284.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Nonlinear optical microscopy techniques can map chemical compositions in biological samples in a label-free manner. Commonly used nonlinear optical processes for imaging include multiphoton excitation fluorescence (MPEF), second harmonic generation (SHG), and coherent Raman scattering (CRS). Femtosecond lasers are typically used for MPEF and SHG due to the requirement of high peak power for excitation, while picosecond lasers are preferred for CRS due to the need for high spectral resolution. Therefore, it is challenging to integrate CRS with MPEF and SHG for chemical imaging. We develop a pulse-picking strategy based on an acousto-optic modulator that can program the duty cycle of the laser pulse train, significantly increasing the pulse peak power at low input average power. This approach offers strong enhancement of nonlinear optical signals and makes hyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy compatible with MPEF and SHG for multimodal imaging at low laser average power. The pulse-picking method also enables the evaluation and comparison of phototoxicity of laser pulses at different average and peak power levels. The photo-perturbations to biological samples are evaluated using cellular dynamics and sample morphological changes, allowing the selection of optimal laser power for the best sensitivity and minimal phototoxicity.
Keyword:
STIMULATED RAMAN-SCATTERING
LIVING CELLS
LIPID DROPLETS
COHERENT
SPECTROSCOPY
EXCITATION
PHOTODAMAGE
DYNAMICS
LASER
VIVO
期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
机构
引用论文
Dynamic Signatures of Lipid Droplets as New Markers to Quantify Cellular Metabolic Changes.png)
ANALYTICAL CHEMISTRY
OAAI
ANALYTICAL CHEMISTRYIF6.7
OAAI分享收藏Fingerprint multiplex CARS at high speed based on supercontinuum generation in bulk media and deep learning spectral denoisingOPTICS EXPRESS
OAAI
OPTICS EXPRESSIF3.3
OAAI分享收藏Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoproteinBIOPHYSICAL JOURNALOAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏Multimodal nonlinear optical microscopy and applications to central nervous system imaging多模态非线性光学显微镜及其在中枢神经系统成像中的应用OAAI
OAAI分享收藏Feature point tracking and trajectory analysis for video imaging in cell biology用于细胞生物学视频成像的特征点跟踪和轨迹分析
PREAI
分享收藏Buckling along boundaries of elastic contrast as a mechanism for early vertebrate morphogenesis
PREAI
分享收藏Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy基于视频速率相干反斯托克斯拉曼散射显微镜的活体组织化学成像OAAI
OAAI分享收藏
查看更多内容