Quantitative DIC microscopy using an off-axis self-interference approach

doi:10.1364/OL.35.002370

2010-07-07

Ramachandra R. Dasari

Michael S. Feld

DOI：10.1364/OL.35.002370

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摘要

En 中文

Traditional Normarski differential interference contrast (DIC) microscopy is a very powerful method for imaging nonstained biological samples. However, one of its major limitations is the nonquantitative nature of the imaging. To overcome this problem, we developed a quantitative DIC microscopy method based on off-axis sample self-interference. The digital holography algorithm is applied to obtain quantitative phase gradients in orthogonal directions, which leads to a quantitative phase image through a spiral integration of the phase gradients. This method is practically simple to implement on any standard microscope without stringent requirements on polarization optics. Optical sectioning can be obtained through enlarged illumination NA. (C) 2010 Optical Society of America

Keyword:

PHASE MICROSCOPY

LIVING CELLS

期刊

Optics Letters

IF:

3.3

论文数:

4.0W

被引数:

7.6W

机构

Korea University

学者数:

3.6W

论文数: 3.8W

被引数: 4.4W

引用论文

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