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Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography
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DOI：10.1364/OE.21.032269.png)
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摘要
En 中文
3-D refractive index (RI) distribution is an intrinsic bio-marker for the chemical and structural information about biological cells. Here we develop an optical diffraction tomography technique for the real-time reconstruction of 3-D RI distribution, employing sparse angle illumination and a graphic processing unit (GPU) implementation. The execution time for the tomographic reconstruction is 0.21 s for 96(3) voxels, which is 17 times faster than that of a conventional approach. We demonstrated the real-time visualization capability with imaging the dynamics of Brownian motion of an anisotropic colloidal dimer and the dynamic shape change in a red blood cell upon shear flow. (C) 2013 Optical Society of America
Keyword:
SPECTROSCOPIC PHASE MICROSCOPY
REFRACTIVE-INDEX
IMAGING TECHNIQUES
HEMOGLOBIN
CELLS
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期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
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