Recent Advances in Endolysin Engineering

M B Aitken

doi:10.3390/antibiotics14121285

2025-12-19

M B Aitken

Gayan S. Abeysekera

Craig Billington *

Renwick C. J. Dobson *

DOI：10.3390/antibiotics14121285

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摘要

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抗菌素耐药性威胁着医学实践中的新“黑暗时代”。慢性抗生素滥用已导致抗菌素耐药性的上升，并促进了多药耐药菌的出现。为解决这一问题，研究人员已开发出新的方法。来源于噬菌体（靶向细菌的病毒）的抗菌剂是极具潜力的候选者。在这些候选者中，噬菌体在病毒复制周期中使用的酶引起了极大的关注。具体而言，溶菌酶被噬菌体用于裂解细菌细胞壁，导致结构崩解和细胞裂解。研究人员正越来越多地将这些蛋白质外用于多药耐药菌，作为一种新型抗菌治疗。随着这一兴趣的增长，许多研究提出了蛋白质工程方法，以进一步强化溶菌酶作为抗菌剂的效能。这些方法包括连接膜透化肽、结构域交换和催化位点修饰。在所有三个领域，近期进展已实现了新型计算机模拟设计流程和基于文库的筛选方法等工具的应用。本综述总结了溶菌酶工程这一快速发展领域中的这些近期进展，并探讨了该领域的潜在未来方向。

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Antibiotics

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University of Canterbury

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6.9K

论文数: 7.1K

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health security group, phf science, christchurch 8041, new zealand

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引用论文

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